Thin Layer Chromatography (TLC) is a simple and cost-effective method used to separate non-volatile compounds in a sample. This technique involves applying a small sample to a thin layer of adsorbent material (such as silica gel) on a flat surface. A solvent (mobile phase) is used to carry the compounds up the plate, separating them based on their interaction with the stationary phase. TLC systems are widely used for qualitative analysis in fields such as pharmaceutical research, chemical synthesis, and food quality testing, providing a quick and reliable method for compound identification.